Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer 2 discriminates Candida parapsilosis, Candida metapsilosis, and Candida orthopsilosis.

نویسندگان

  • Andrew M Borman
  • Christopher J Linton
  • Debra Oliver
  • Michael D Palmer
  • Adrien Szekely
  • Frank C Odds
  • Elizabeth M Johnson
چکیده

Two new cryptic sister species, Candida orthopsilosis and Candida metapsilosis, were recently identified by consistent DNA sequence differences among several genes within the genetically heterogeneous Candida parapsilosis complex. Here, we present data demonstrating that Pyrosequencing analysis of 20 nucleotides of internal transcribed spacer region 2 rapidly and robustly distinguishes between these three closely related Candida species.

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منابع مشابه

Pyrosequencing ® analysis of 20 nucleotides of the 1 Internal Transcribed

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 47 7  شماره 

صفحات  -

تاریخ انتشار 2009